Performance of BinaxNOW G6PD deficiency point-of-care diagnostic in P. vivax-infected subjects
Title | Performance of BinaxNOW G6PD deficiency point-of-care diagnostic in P. vivax-infected subjects |
Publication Type | Journal Article |
Year of Publication | 2015 |
Authors | Osorio, L, Carter, N, Arthur, P, Bancone, G, Gopalan, S, Gupta, SK, Noedl, H, Kochar, SK, Kochar, DK, Krudsood, S, Lacerda, MV, Llanos-Cuentas, A, Rueangweerayut, R, Srinivasan, R, Treiber, M, Mohrle, JJ, Green, J |
Journal | Am J Trop Med Hyg |
Volume | 92 |
Issue | 1 |
Pagination | 22-7 |
Date Published | Jan |
ISBN Number | 1476-1645 (Electronic)0002-9637 (Linking) |
Keywords | *Point-of-Care Systems, Antimalarials/*therapeutic use, Glycogen Storage Disease Type I/*diagnosis, Humans, Malaria, Vivax/*drug therapy, Sensitivity and Specificity |
Abstract | Accurate diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency is required to avoid the risk of acute hemolysis associated with 8-aminoquinoline treatment. The performance of the BinaxNOW G6PD test compared with the quantitative spectrophotometric analysis of G6PD activity was assessed in 356 Plasmodium vivax-infected subjects in Brazil, Peru, Thailand, and India. In the quantitative assay, the median G6PD activity was 8.81 U/g hemoglobin (range = 0.05-20.19), with 11 (3%) subjects identified as deficient. Sensitivity of the BinaxNOW G6PD to detect deficient subjects was 54.5% (6 of 11), and specificity was 100% (345 of 345). Room temperatures inadvertently falling outside the range required to perform the rapid test (18-25 degrees C) together with subtlety of color change and insufficient training could partially explain the low sensitivity found. Ensuring safe use of 8-aminoquinolines depends on additional development of simple, highly sensitive G6PD deficiency diagnostic tests suitable for routine use in malaria-endemic areas. |
URL | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4347383/pdf/tropmed-92-22.pdf |
Short Title | The American journal of tropical medicine and hygiene |