Diagnostic Accuracy Assessment of Immunochromatographic Tests for the Rapid Detection of Antibodies Against Orientia tsutsugamushi Using Paired Acute and Convalescent Specimens
Title | Diagnostic Accuracy Assessment of Immunochromatographic Tests for the Rapid Detection of Antibodies Against Orientia tsutsugamushi Using Paired Acute and Convalescent Specimens |
Publication Type | Journal Article |
Year of Publication | 2015 |
Authors | Watthanaworawit, W, Turner, P, Turner, C, Tanganuchitcharnchai, A, Jintaworn, S, Hanboonkunupakarn, B, Richards, AL, Day, NP, Blacksell, SD, Nosten, F |
Journal | Am J Trop Med Hyg |
Volume | 93 |
Issue | 6 |
Pagination | 1168-71 |
Date Published | Dec 9 |
ISBN Number | 1476-1645 (Electronic)0002-9637 (Linking) |
Abstract | We assessed the diagnostic accuracy of two immunochromatographic tests (ICTs), the Access Bio CareStart Scrub Typhus test (Somerset, NJ) (IgM), and the SD BIOLINE Tsutsugamushi test (Kyonggi-do, Republic of Korea) (IgG, IgM, or IgA) compared with indirect immunofluorescence assay (IFA) and real-time PCR results as reference tests using 86 paired acute and convalescent specimens from febrile patients. The sensitivity and specificity of the CareStart test were 23.3% (95% confidence interval [CI]: 11.8-38.6) and 81.4% (95% CI: 66.6-91.6), respectively, for acute specimens and 32.6% (95% CI: 19.1-48.5) and 79.1% (95% CI: 64.0-90.0), respectively, for convalescent specimens. For the SD BIOLINE test, sensitivity and specificity were 20.9% (95% CI: 10.0-36.0) and 74.4% (95% CI: 58.8-86.5), respectively, for acute specimens and 76.7% (95% CI: 61.4-88.2) and 76.7% (95% CI: 61.4-88.2), respectively, for convalescent specimens. The poor sensitivity obtained for both ICTs during this study when performed on acute specimens highlights the difficulties in prompt diagnosis of scrub typhus. |
URL | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674230/pdf/tropmed-93-1168.pdf |